KRTAP4-16 Knockout cell line (A549)

KRTAP4-16 Knockout cell line (A549) Order

Catalog#

YKO-XN67889

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

KRTAP4-16 Knockout cell line (A549)

Cell Morphology

Epithelial-like, adherent

Passage ratio

1:3~1:4

Species

Human

Gene Name

KRTAP4-16

Gene ID

85354

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90% F12K+10% FBS

Target region

Knockout region: Exon 1 (based on transcript KRTAP4-16-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:A549			STR Info (Cell bank) Cell Line:A-549
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	11		11	11
D13S317	11	11		11	11
D7S820	8	11		8	11
D16S539	11	12		11	12
VWA	14	14		14	14
TH01	8	9.3		8	9.3
AMEL	X	Y		X	Y
TPOX	8	11		8	11
CSF1PO	10	12		10	12
D12S391	18	18
FGA	23	23
D2S1338	24	24
D21S11	29	29
D18S51	14	17
D8S1179	13	14
D3S1358	16	16
D6S1043	11	13
PENTAE	7	11
D19S433	13	13
PENTAD	9	9
D1S1656	17	18.3

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the KRTAP4-16 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the A549 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, KRTAP4-16 gene knockout KRTAP4-16 Knockout cell line (A549) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

KRTAP4-16 Knockout cell line (A549)

Catalog#

YKO-XN67889

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Related Popular Products

Wild-type Cell Lines

Human Non-small Cell Lung Cancer Cell Line(A549)
inquiry More details

Catalog#: YC-C016

Size: 1*10^6

Instruction:

Gene Knockout kit

EZ-editor™Gene Knockout kit
inquiry More details

Catalog#: MY-001

Size: -

Instruction:

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium
inquiry More details

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

KO Cell Lines

KRTAP4-16 Knockout cell line (Hep G2)
inquiry More details

Organism: Human

Gene Name: KRTAP4-16

Gene ID: 85354

Size: 1*10^6

Catalog#: YKO-HT7660

YKO-HT7660(Hep G2)
KRTAP4-16 Knockout cell line (HeLa)
inquiry More details

Organism: Human

Gene Name: KRTAP4-16

Gene ID: 85354

Size: 1*10^6

Catalog#: YKO-XN67892

YKO-XN67892(HeLa)
KRTAP4-16 Knockout cell line (HCT 116)
inquiry More details

Organism: Human

Gene Name: KRTAP4-16

Gene ID: 85354

Size: 1*10^6

Catalog#: YKO-XN67891

YKO-XN67891(HCT 116)

Related Popular Products

Wild-type Cell Lines

Human Non-small Cell Lung Cancer Cell Line(A549)

Price (USD): -

Catalog#: YC-C016

Size: 1*10^6

Instruction:

inquiry More details

Gene Knockout kit

EZ-editor™Gene Knockout kit

Price (USD): 998

Catalog#: MY-001

Size: -

Instruction:

inquiry More details

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium

Price (USD): -;-

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

inquiry More details

KO Cell Lines

KRTAP4-16 Knockout cell line (Hep G2)

Organism: Human

Gene Name: KRTAP4-16

Gene ID: 85354

Size: 1*10^6

Catalog#: YKO-HT7660

inquiry More details
KRTAP4-16 Knockout cell line (HeLa)

Organism: Human

Gene Name: KRTAP4-16

Gene ID: 85354

Size: 1*10^6

Catalog#: YKO-XN67892

inquiry More details
KRTAP4-16 Knockout cell line (HCT 116)

Organism: Human

Gene Name: KRTAP4-16

Gene ID: 85354

Size: 1*10^6

Catalog#: YKO-XN67891

inquiry More details

KRTAP4-16 Knockout cell line (A549)

Ubigene